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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
18/12/2018 |
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Data da última atualização: |
18/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PINHEIRO, A. F.; ROLOFF, B. C.; MOREIRA, A. da S.; BERNE, M. E. A.; ANDREOTTI, R.; LEITE, F. P. L. |
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Afiliação: |
Amanda Fernandes Pinheiro, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas - UFPel; Bárbara Couto Roloff, Núcleo de Tecnologia/Centro de Desenvolvimento Tecnológico/Universidade Federal de Pelotas - UFPel; Angelita da Silveira Moreira, Núcleo de Tecnologia/Centro de Desenvolvimento Tecnológico/Universidade Federal de Pelotas - UFPel; Maria Elisabeth Aires Berne, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas - UFPel; RENATO ANDREOTTI E SILVA, CNPGC; Fábio Pereira Leivas Leite, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas – UFPel. |
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Título: |
Identification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Vaccine, v. 36, n. 9, p. 1154-1159, February 2018 |
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Idioma: |
Inglês |
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Conteúdo: |
The parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In conclusion, it was found that the association of the recombinant prNcSRS2 protein with different adjuvants induced different levels of specific antibody, and a distinct splenic cytokine profile in an adjuvant-dependent manner. The mechanisms of adjuvancity activity is complex, so adjuvant formulation may help in the design of efficient vaccine to control Neosporosis. MenosThe parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In concl... Mostrar Tudo |
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Thesagro: |
Neospora Caninum. |
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Thesaurus Nal: |
Adjuvants; Cytokines; Proteins; Vaccines. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188808/1/Identification-of-suitable-adjuvant-for-vaccine.pdf
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Marc: |
LEADER 02555naa a2200241 a 4500
001 2101958
005 2018-12-18
008 2018 bl uuuu u00u1 u #d
100 1 $aPINHEIRO, A. F.
245 $aIdentification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2.$h[electronic resource]
260 $c2018
520 $aThe parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In conclusion, it was found that the association of the recombinant prNcSRS2 protein with different adjuvants induced different levels of specific antibody, and a distinct splenic cytokine profile in an adjuvant-dependent manner. The mechanisms of adjuvancity activity is complex, so adjuvant formulation may help in the design of efficient vaccine to control Neosporosis.
650 $aAdjuvants
650 $aCytokines
650 $aProteins
650 $aVaccines
650 $aNeospora Caninum
700 1 $aROLOFF, B. C.
700 1 $aMOREIRA, A. da S.
700 1 $aBERNE, M. E. A.
700 1 $aANDREOTTI, R.
700 1 $aLEITE, F. P. L.
773 $tVaccine$gv. 36, n. 9, p. 1154-1159, February 2018
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 14 | |
| 5. |  | SIVIERO, A.; SANTOS, F. A.; CRISTOFANI, M.; FURTADO, E. L.; MACHADO, M. A. Avaliação in vitro de genótipos de citros a Phytophthora parasitica. Fitopatologia Brasileira, Brasília, DF, v. 29, n. 3, p. 300-302, maio/jun. 2004.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Acre. |
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| 6. | | BASTIANEL, M.; OLIVEIRA, A. C. de; CRISTOFANI, M.; MACHADO, M. A. Diversidade genética entre híbridos de laranja-doce e tangor 'Murcott' avaliada por fAFLP e RAPD. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 5, p. 779-784, maio 2006 Título em inglês: Genetic diversity among hybrids of sweet orange and ?Murcott? tangor evaluated by fAFLP and RAPD markers.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 7. | | OLIVEIRA, R. P. de; CRISTOFANI, M.; AGUILAR-VILDOSO, C. I.; MACHADO, M. A. Diversidade genética entre híbridos de tangerina 'Cravo' e laranja 'Pêra'. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 4, p. 479-484, abr. 2002 Título em inglês: Genetic diversity among hybrids of "Cravo" mandarin and 'Pêra' sweet orange.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 8. | | CRISTOFANI, M.; SOUZA, A. A.; KISHI, L.; TAKITA, M. A.; TARGON, M. L. P. N.; MACHADO, M. A. Genes diferencialmente expressos em híbridos de Citrus sunki vs Poncirus trifoliata resistentes ao Citrus tristeza virus detectados por hibridização subtrativa. Fitopatologia Brasileira, Brasília, DF, v. 29, p. S223, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | LUCHETA, A. R.; SILVA-PINHATI, A. C. O.; BASÍLIO-PALMIERI, A. C.; BERGER, I. J.; FREITAS-ÁSTUA, J.; CRISTOFANI, M. An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 819-831, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | CRISTOFANI, m.; NOVELLI, V. M.; PERIN, M. S.; OLIVEIRA, A. C. de; OLIVEIRA, R. P. de; BASTIANEL, M.; MACHADO, M. A. Programa de melhoramento de citros por hibridação controlada no centro apta citros "Sylvio Moreira"/IAC em 1997-2005. LARANJA, Cordeirópolis, v.26, n.1, p.121-134, 2005. 14 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 11. | | MACHADO, M. A.; ASTUA-MONGE, G.; TARGON, M. L. P. N.; TAKITA, M. A.; FREITAS-ASTUA, J.; SOUZA, A. A.; COLETA FILHO, H. D.; AMARAL, A. M. do; CRISTOFANI, M.; BASTIANEL, M. Citrus genomics in Brazil (CitEST): experiences and future perspectives. In: PLANT & ANIMAL GENOME, 13., 2005, San Diego, USA. Abstracts... San Diego: Scherago International, 2005. p. 18| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | MACHADO, M. A.; ASTUA-MONGE, G.; TARGON, M. L. P. N.; TAKITA, M. A.; FREITAS-ASTUA, J.; SOUZA, A. A.; COLETTA FILHO, H. D.; AMARAL, A. M. do; CRISTOFANI, M.; BASTIANEL, M.; PALMIERI, D. A.; BASILIO, A. C. Citrus genomics in Brazil (CitEST): experiences and future perspectives. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 34., 2005, Águas de Lindóia. Programa e índices... Águas de Lindóia: SBBq, 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | SOUZA, A. A. de; TARGON, M. L. P. N.; KISHI, L. T.; ROSA, D. D.; CAMPOS, M. A.; ASTUA MONGE, G.; CRISTOFANI, M.; TAKITA, M. A.; MACHADO, M. A. Genes de Phytophthora parasitica relacionados à degradação da parede celular vegetal isolados por biblioteca de cDNA. Fitopatologia Brasileira, Brasília, DF, v. 29, p. S200, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | SOUZA, A. A.; TAKITA, M. A.; COLETTA FILHO, H. D.; TARGON, M. L. P. N.; RODRIGUES, C. M.; CARLOS, E. F.; AMARAL, A. M.; CRISTOFANI, M.; TEIXEIRA, J. E. C.; ROSA, D. D.; CAMPOS, M.; OLIVEIRA, A. C.; OLIVEIRA, R. C. de; NUNES, L. R.; FERRAZ, J. R.; FISCHER, C. N.; MACHADO, M. A. Comparative analysis of expressed sequence tags in Citrus reticulata infected with Xylella fastidiosa. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. A era da genômica: da bioestatística à bioinformática: anais. Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2005. p. 490.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 14 | |
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